lipL [2019-09-06 08:48:54]

N-amidotransferase, transfers lipoic acid from lipoyl-GcvH to AcoC, BkdB, OdhB, and PdhC, and from lipoyl-OdhB to BkdB and PdhC

Locus: BSU_37640

Isoelectric point: 5.27

Molecular weight: 31.27 kDa

Protein length: 281 aa

Gene length: 846 bp

Function: lipoic acid metabolism

Product: protein N-octanoyltransferase

Essential: no

E.C.: 2.3.1.204

Synonyms: ipa-90d,ywfL

Genomic Context

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Categories containing this gene/protein

• Metabolism → Additional metabolic pathways → Biosynthesis of cofactors → Biosynthesis and scavenging of lipoic acid

Gene

Coordinates: 3,863,415 → 3,864,260

The protein

Catalyzed reaction/ biological activity

• transfers lipoic acid from lipoyl-GcvH to BkdB, OdhB, and PdhC, and from lipoyl-OdhB to BkdB and PdhC PubMed
• [glycine-cleavage complex H protein]-N⁶-octanoyl-L-lysine + [lipoyl-carrier protein]-L-lysine --> [glycine-cleavage complex H protein]-L-lysine + [lipoyl-carrier protein]-N⁶-octanoyl-L-lysine (according to UniProt)

Protein family

• Octanoyltransferase LipL family (single member, according to UniProt)

Structure

• 2P5I (from Bacillus halodurans, 56% identity)

Expression and Regulation

Operons

• Genes: lipL
  Description: PubMed

Biological materials

Mutant

• MGNA-B667 (ywfL::erm), available at the NBRP B. subtilis, Japan
• BKE37640 (ΔlipL::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCTATGTAAAACCCTTTC, downstream forward: _UP4_TGAACGTTCTTCGCATGCCG
• BKK37640 (ΔlipL::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCTATGTAAAACCCTTTC, downstream forward: _UP4_TGAACGTTCTTCGCATGCCG

References

Reviews

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Original publications

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